use of genetically-engineered cells to produce a vaccine for malaria

1.Your research group is investigating the possible use of

genetically-engineered cells to produce a vaccine for malaria. List

the pros and cons of using E. coli, Saccharomyces, and animal cells to

produce the vaccine.

2.The local public health agency has received reports of an outbreak of

Salmonella gastroenteritis among attendees at a city-sponsored chili

cook-off. What techniques from recombinant DNA technology would the

agency likely use to investigate this outbreak? Describe the expected

results from these techniques.

3. Pseudomonas syringae is found naturally in the soil. Sold as Snomax,

it is used to make snow at ski resorts. The same bacterium with a gene

deletion (Ice-minus) is used to prevent ice formation on plants.

Should Snomax and Ice-minus be considered modified organisms and

subject to precautions of releasing genetically modified organisms?

Explain why or why not.

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