What is the difference between one channel and two channel microarrays, and what might be the important advantage of using 2 channel microarrays?
Two channel microarrays enables a researcher to investigate or view two specimens at the same time, hence, making him to draw any differences amid the two of which are of the similar type. The system ensures that the intended comparison amid two given samples the researcher draw distinct features that may seem contradicting the other. Conversely, one channel microarray, only a single observation within a given timeframe where the researcher does the experiment to its completion while recording the observable changes prior observing a second sample. Then comparison and conclusions follow with the drawing of the main conclusions. Data and information obtained in a single or one channel microarray are exaggerated and it may posse a challenge in drawings comparisons while two channel its features seem normal. Since the differing features comes from the highlighted fields which are meant for comparison.
2. Describe the difference between gene expression, exon, tiling and promotor arrays.
Tilling arrays
They are high intensity subtype microarrays that mainly applied in mapping used in studying solid surfaces requiring clear illumination. They differ with other arrays in the nature of probes since they do posses very short fragments designed to foe entire coverage.
Gene expression arrays
This array in comparison to the three arrays poses very limited spacing in their diagrammatic representation. Hence, implying lowest intensity plus diminutive strength to study and retrieve information from solid surfaces. The intensity of gene array increases towards the end and mainly their application is in measuring the levels of large population of genes.
Exon arrays
The diagrammatic representation of these arrays indicates evenly distribution of spacing of the three in each band implying uniform intensity all through the illumination on the surface of the study. They do allow a platform to research large-scale variants.
4. State two advantages of using the Fluidigm BioMark rather than standard real-time PCR
Fluidigm BioMark undertakes real time PCR reactions in a high meticulous manner
Fluidigm BioMark is able to carry out numerous PCR reactions (approximately 30,000 per day) in one single run.
Question 5.
a) mRNA sequencing
It is high throughput sequencing know which is dilapidating with time currently due to incoming of new and better technological knowhow in medical fields. Mainly its applications enable researchers to obtain information regarding RNA sample content. In addition, it allows researchers to obtain transscrptome data experimentally.
b) deNovo sequencing
It is a sequencing experiment executed with no prior information of amino acid sequence. Edman degradation practitioners execute mainly this experiment daily with high doubts on million-dollar spectrometer that lies idle but can undertake the task in a few seconds. This experiment entails sequencing peptides in days especially in the absence of mass and complicated spectrometer.
Question 6
a) According to Human Genome Project studies, the haploid human genome approximately occupies more than DNA base pairs. In addition, it is composed of 23, 000 protein-coding genes. (it is correct).
b) Only 30 times coverage is essential in sequencing human genome i.e
2*100bp = 200bp (which are doubled ends)* 250 million (sequence per lane)
= 5×1010 bp, hence 16.67 / lane
Therefore = 30/16.67
= 1.7996 lanes.
c) The number of lanes is 1.7996 lanes. As calculated above.
Both questions I assume the similar conditions and criterion, i.e 100bp and 200bp (which are doubled ends).
Hence the correlation,
2*100bp = 200bp (which are doubled ends)* 250 million (sequence per lane)
