Project Paper (15%), scientific record (<1,500 words) of methodology, results and outcomes of project activity, up to and including week 13 activity.
Due: Friday 22May (6pm), submitted via Turnitin.
The Project Paper should consist of:
• Opening sentence is ‘literary hook’ to captivate reader
• First few sentences need to convey significance of the research effort (i.e. in global/regional/local terms).
• Remainder of paragraph one outlines context (i.e. background material).
• Middle paragraphs elaborate and convey relevant details of the methodology and data analysis, in concise form.
• Title, Headings (Introduction, Materials, Methods, Results, Discussion and References) should be used.
• Information and format should be consistent with Cell Biology based scientific journals.
• The interpretation and critical analysis (identifying the Pros & Cons for any arguments) must be cogent and conveyed in an informed and balanced manner.
• Concluding statements must reiterate (not in the same words) the significance of the research effort and outcomes.
• Final statement needs to indicate the direction you believe the current research is likely to go (based on current scientific/technology information).
Resources
These Web link maybe useful
http://jcb.rupress.org/content/165/6/757.full
https://www.colby.edu/biology/BI17x/writing_papers.html
Rubric: Project Abstract and Paper Criteria Unsatisfactory Pass Credit Distinction High Distinction
Evidence Does not apply report to topic and/or demonstrate the use of e-journals and/or demonstrate accurate knowledge and/or provide applied examples. Applicable to the topic. Demonstrates the use of e-journals. Demonstrates accurate knowledge and provides applied examples. Focused on the topic. Demonstrates comprehensive use of e-journals and knowledge with the pertinent use of applied examples. Focussed on the topic drawing out key aspects. Demonstrates comprehensive and informative use of e-journals with the use of informed knowledge and thought provoking applied examples. Perceptive application to the topic enlarging on key aspects. Demonstrates perceptive use of e-journals with the use of insightful knowledge and original examples.
Format Not clear and to the point and/or logically sequenced and/or does not cover the whole topic and/or equitable contributions. Clear and to the point and/or logically sequenced and/or covers the whole topic with equitable contributions. Clear to the point. Logically sequenced with comprehensive explanations and transition. Comprehensively covers the whole topic. Seamless transitions that enable equitable contributions Clear to the point. Logically sequenced with comprehensive thought provoking explanations and precise transitions from point to point. Informatively covers the whole topic enabling equitable contributions. Clear to the point. Logically sequenced with insightful explanations and concise transitions from point to point. Perceptive coverage of the whole topic. Seamless and insightful discussion while still enabling equitable contributions.
Analysis No argument presented and/or logical order and/or not consistent, readable, brief, clear, and to the point and/or not all sources/direct quotes cited correctly and/no evidence of spell/grammar check. Argument presented and or logically ordered. Consistent, readable, brief, clear and to the point. Correct citing of sources and direct quotes. Spelling and grammar correct. Argument comprehensive, logically and visually thoroughly presented. Enhanced with the use of illustrations and/or graphics. Correct citing of all sources and direct quotes. Spelling and grammar correct. Argument thought provoking, logically and succinctly developed. Enhanced and professional with the use of illustrations, captions and/or graphics. Correct citing of all sources and direct quotes. Spelling and grammar correct. Argument insightful, logically and succinctly developed. Enhanced, professional with the use of original ideas, illustrations and/or graphics that broaden understanding of the topic. Correct citing of all sources and direct quotes. Spelling and grammar
Project Aim, Milestones and Objectives
Aim:
Characterise the RNA, DNA and Protein (Actin) response of progenitor stems cells (L6 Myoblasts) to Transcription Inhibitors (TI’s).
Overview:
Investigators required to formulate a hypothesis to test, by developing protocols for culture, treatment and characterization of, progenitor stem cell responses to transcription inhibition.
Materials/Resources:
• Culture plate (6 well) & Fluro Dish (glass bottom culture dish).
• DMEM Culture media and Trypan Blue (cell viability stain).
• Bright field stain: Brilliant Cresyl Blue (BCB), 10 % stock in water.
• Fluorescent stain: Actin green (filamentous Actin)
• Treatment – each team member assigned one TI (supplied @ 1mM/100μL). o Actinomycin D (Actino),
o Triptolide (Trip),
• Leica microscope software (downloads via vUWS).
• Confocal (Fluorescent) data (supplied to all investigators*): o DNA Stain – NucBlue
o RNA Stain – Pyronin Y
o Protein Stain – Actin Green
Methods:
1. Each investigator to develop their own L6 cell culture and BCB staining protocol.
2. Each investigator to develop their own TI treatment regime on L6 cells.
3. Acquire, process and analyse BCB and FS data (using Leica software) following TI treatment.
4. Acquire, process and analyse Confocal data (provided*).
Milestones (Project Lab Book):
1. Reference Material & Procedures (2%): Due week 2. 1.1. Trypan Material Safety Data Sheet (MSDS#) and summary in Laboratory Notebook.
1.2. Myoblast (L6) cell culture information, BCB and TI details recorded in Lab Notebook.
1.3. Live cell counts (per ml) recorded in Laboratory Notebook.
2. Experiment Procedure& Bright Field Stain Preparation (2%): Due week 3. 2.1. Standard Operating Procedure (SOP) submitted via Turnitin, recorded in Laboratory Notebook.
2.2. Prepare 6 well plates with cells and treat with TI.
3. Data & Analysis (2%): Due week 5. 3.1. Control and Treated cell viability tabulated in Laboratory Notebook.
3.2. BCB stain data acquired and analysed (electronic or hardcopy).
4. Confocal Analysis & Experiment Design -Actin Stain (2%): Due week 10. 4.1. Formalin MSDS# and summary in Laboratory Notebook.
4.2. Confocal data acquired in weeks 6-8 analysed (electronic or hardcopy).
4.3. Control Fluro dish fixing and Actin Staining protocol recorded in Laboratory Notebook.
5. Data & Analysis (2%): Due week 12. 5.1. Treated and Actin stained cell data acquired and analysed (electronic or hardcopy).
Objectives:
1. Project Abstract (5%): Concise interim report of methodology and interim results.
Due Friday 10 Apr (6pm), submitted via Turnitin.
2. Project Paper (15%): Scientific record addressing materials, methods, results and discussion/outcomes of project, with references.
Due Friday 22 May (6pm), submitted via Turnitin.
